An assignment Essay Example | Topics and Well Written Essays - 750 words

An assignment - Essay Example The equipment in the trauma unit must be suitable for trauma imaging, and consequently, the radiographers must be aware of the differences between a trauma imaging unit and a general imaging unit. In a range of client groups, the techniques should be suitably adapted, and the equipment must permit this. The radiographers should be able to analyse trauma images, and if they deliver inadequate information, they must suggest complementary investigations to aid diagnosis and treatment. The anatomical knowledge should be thorough, so depending on the target area of imaging, there may be need for adaptation of position, and during trauma imaging, the radiographer should demonstrate these skills. Trauma patients are exposed to the risk of changing clinical situations even during imaging, and the trauma radiographer must demonstrate awareness about the dynamics of a changing situation during imaging (Murray et al., 2008, 289) Taking a detailed history and examination of the patient may provide a working diagnosis, so the radiographer is able to understand and deduce the mechanism of injury. In some cases due consideration must be given to the fact that sometimes imaging may not add value to the management of the condition but may aggravate the situation due to manipulation during imaging. In most cases there is significant time penalty, and thus delay due to unnecessary imaging may be avoided. Additionally, performing an imaging in an inappropriate indication leads to the breach of laws related to radiation exposure. Using the knowledge of anatomy and possible mechanism of trauma would lead to correct area, correct positioning, and correct view of the image. Sometimes, this approach would lead to a prediction about further views. It is important to focus on the correct area for imaging since large field of views may cause missing of subtle injuries. When a fracture is suspected always two views

Mass Spectrometry and HPLC of peptides Lab Report

Mass Spectrometry and HPLC of peptides - Lab Report Example The mass of the protonated and solvated molecules pass through the mass analyzer that establishes the mass of the analyte. Reversed phase high pressure liquid chromatography relies on the reversible water-hating associations between the molecules of the solute present in a polar mobile phase and the immobilised hydrophobic ligand in the solid support to effect separation (David 2001). The dispersal of the solute between the two phases is a function of the binding attributes of the medium, the polarity of the solute and the constituents of the mobile phase. The polarity of the mobile phase is lowered by the addition of organic solvents, which minimize the hydrophobic associations between the solute and the stationary phase hence causing desorption. Hydrophobic molecules end up spending more time on the solid support hence requiring a high solvent concentration to promote desorption. The mass to charge ratio (m/z) is the proportion of the mass of a substance to the number of ions present in a substance. This value is obtained sing the formula m/z = (MW + nH+)/n where m/z = the mass-to-charge ratio, which is indicated on the axis of the spectrum; MW is the molecular mass of the sample; n is the integer number of charges on the ions and H is the mass of a proton, which is equivalent to 1.008 Da. The above equation can be used to find the molecular weight of a sample provided that the sum of charges on an ion is established. The m/z value is read from the spectrum and substituted into the equation. Usually, during the calculation of charge, it is assumed that any two adjacent members in the sequence of multiplied charged ions differ by one charge. The mass of charge ratio (m/z) of large molecules such as ionised proteins, for example, lysozyme and bovine serum albumin is obtained in the same manner as described. The charge of the ion is found by monitoring adjacent charge states and substituted into the above equation alongside the m/z ratio that